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Assay Principle for the TBARS Parameter Kit. Acid-treated samples and standards, followed by the TBA reagent, are added to the included 96-well microplate ( Step 1 ). The microplate is then incubated at 45-50 C for 2-3 hours, during which time the MDA in the sample reacts with the TBA reagent to produce a colored end product ( Step 2 ).
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The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured.
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